Howard S G, Garcia-Rill E
Brain Res Bull. 1983 Apr;10(4):437-40. doi: 10.1016/0361-9230(83)90140-5.
The concentration of endogenous- and deuterium-labeled acetylcholine (ACh) in the cat caudate nucleus was determined after stimulation of either the substantia nigra or the precruciate cortex. In this procedure the caudate nucleus is exposed surgically, and a coring device is used to obtain biopsy specimens which are immediately frozen in liquid nitrogen. Samples are collected at rest, 5 min after stimulation, and again 5 min after a resting period. An infusion of 2H9-choline is maintained during these manipulations to provide a label for ACh synthesis. Electrical stimulation of the substantia nigra, which increases the release of dopamine, produced a decrease in endogenous ACh and the newly synthesized deuterium-labeled ACh. Stimulation of the precruciate cortex produced no significant effect on the levels or synthesis of ACh, but attenuated the effect of subsequent nigral stimulation. These preliminary results indicate that stimulation of the substantia nigra has a net excitatory effect on ACh synthesis in the caudate. This stimulation apparently is modulated by input from the cortex.
在刺激猫的黑质或十字前皮质后，测定了猫尾状核中内源性和氘标记的乙酰胆碱（ACh）浓度。在此过程中，通过手术暴露尾状核，并用取芯装置获取活检标本，这些标本立即在液氮中冷冻。在静息状态、刺激后5分钟以及休息一段时间后再次刺激后5分钟采集样本。在这些操作过程中持续输注2H9-胆碱，以提供ACh合成的标记。电刺激黑质会增加多巴胺的释放，导致内源性ACh和新合成的氘标记ACh减少。刺激十字前皮质对ACh的水平或合成没有显著影响，但减弱了随后黑质刺激的效果。这些初步结果表明，刺激黑质对尾状核中ACh的合成具有净兴奋作用。这种刺激显然受到来自皮质输入的调节。
