Gilad G M, Gilad V H
Exp Neurol. 1983 Jul;81(1):158-66. doi: 10.1016/0014-4886(83)90165-6.
The activity of ornithine decarboxylase (ODC) was examined in the rat superior cervical ganglion during development and after postganglionic axotomy. During development the enzyme activity in the ganglion was transiently elevated at 19 days gestation (2 days before birth) and then declined to low adult activities by 5 days after birth. After axotomy the enzyme activity in the ganglion was rapidly increased. As early as 2 h after the injury the activity was increased to 200% and by 10 h it attained a peak of 453% above control values. The activity then rapidly declined to a lower value of about 200% above control by 24 h, where it remained as long as 5 days and then returned to control values by 10 days postoperative. The early increase in ODC activity was demonstrated by inhibitor cytochemistry to occur within neuronal cell bodies. The ODC activity was also increased after preganglionic nerve transection (denervation) (275%), end organ removal (60%), and 6-hydroxydopamine treatment (75%). We conclude that increased ODC activity is one of the earliest events in the reaction of the nerve cell body to axonal injury, and that increased ODC activity is a common feature of drastic metabolic alterations in neurons of the superior cervical ganglion.
在发育过程中以及节后轴突切断后，对大鼠颈上神经节中的鸟氨酸脱羧酶（ODC）活性进行了检测。在发育过程中，神经节中的酶活性在妊娠19天（出生前2天）时短暂升高，然后在出生后5天降至成年后的低水平。轴突切断后，神经节中的酶活性迅速增加。早在损伤后2小时，活性就增加到对照值的200%，到10小时时达到比对照值高453%的峰值。然后活性迅速下降，到24小时时降至比对照值高约200%的较低水平，并在此水平维持长达5天，然后在术后10天恢复到对照值。通过抑制剂细胞化学证明，ODC活性的早期增加发生在神经元细胞体内。节前神经切断（去神经支配）（275%）、终末器官切除（60%）和6-羟基多巴胺处理（75%）后ODC活性也增加。我们得出结论，ODC活性增加是神经细胞体对轴突损伤反应中最早出现的事件之一，并且ODC活性增加是颈上神经节神经元剧烈代谢改变的一个共同特征。
