Prosser J S, Moquet J E
Experientia. 1983 Jul 15;39(7):778-80. doi: 10.1007/BF01990323.
Human blood was stored for up to 3 days before 66-h culture with 3 concentrations of bromodeoxyuridine (BrdU) (10, 20 and 40 microM). In cultures containing 10 or 20 microM BrdU, the number of cells staining as first division metaphases (MI) increased with storage time. In addition, as the storage time was increased the staining quality deteriorated although this was less pronounced in cultures containing 40 microM BrdU. The implications of these results for routine fluorescence plus Giemsa staining as part of a cytogenetic radiation dosimetry service are discussed, with the recommendation that for accurate identification of M1 cells, the concentration of BrdU should be increased in cultures set up from blood that has been stored for more than 2 days.
在含有3种浓度（10、20和40微摩尔）溴脱氧尿苷（BrdU）的条件下进行66小时培养之前，将人血储存长达3天。在含有10或20微摩尔BrdU的培养物中，作为第一次分裂中期（MⅠ）染色的细胞数量随储存时间增加。此外，随着储存时间延长，染色质量下降，不过在含有40微摩尔BrdU的培养物中这种情况不太明显。讨论了这些结果对作为细胞遗传辐射剂量测定服务一部分的常规荧光加吉姆萨染色的影响，并建议为准确鉴定M1细胞，对于从储存超过2天的血液中建立的培养物，应提高BrdU的浓度。
