鸡降钙素信使核糖核酸的无细胞翻译

Cell free translation of chicken calcitonin messenger RNA.

作者信息

Lasmoles F, Desplan C, Segond N, Jullienne A, Treilhou-Lahille F, Milhaud G, Moukhtar M S

出版信息

FEBS Lett. 1983 Jun 27;157(1):100-4. doi: 10.1016/0014-5793(83)81124-7.

PMID:6862006

Abstract

The primary step of calcitonin biosynthesis was studied in a normal organ: chicken ultimobranchial gland, a tissue particularly rich in calcitonin secretory cells. Poly(A)-rich RNA was extracted and purified from ultimobranchial organs and translated in a reticulocyte lysate in the presence of labelled methionine. Polyacrylamide gel electrophoresis of specific immunoprecipitates revealed a major band of Mr 14 500 and a band of Mr 13 300. Thus, in chicken the precursor of calcitonin is a Mr 14 500 polypeptide. The minor component of Mr 13 300 could represent limited processing by the reticulocyte lysate.

摘要

降钙素生物合成的第一步在一个正常器官中进行了研究

鸡的 ultimobranchial 腺，一种富含降钙素分泌细胞的组织。从 ultimobranchial 器官中提取并纯化了富含 poly(A) 的 RNA，并在存在标记甲硫氨酸的情况下在网织红细胞裂解物中进行翻译。特异性免疫沉淀物的聚丙烯酰胺凝胶电泳显示出一条主要的 Mr 14500 条带和一条 Mr 13300 条带。因此，在鸡中，降钙素的前体是一种 Mr 14500 的多肽。Mr 13300 的次要成分可能代表网织红细胞裂解物的有限加工。