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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Leissing N C, McGuinness E T

Int J Biochem. 1983;15(5):651-6. doi: 10.1016/0020-711x(83)90189-1.

A steady state kinetic investigation was performed on an improved preparation of rat-liver sorbitol dehydrogenase (L-iditol: NAD-oxidoreductase, EC 1.1.1.14). 2. Data analyses indicate the enzyme follows a rapid equilibrium random mechanism in the direction of sorbitol oxidation and a random mechanism in the direction of fructose reduction. 3. Kinetic constants were: K m NAD 0.082 mM; K m sorbitol 0.38 mM; K m NADH 67 microns; K m fructose 136 mM. 4. Evidence is adduced to indicate the more rapid reverse (fructose reduction) reaction is susceptible to metabolic control by formation of abortive enzyme fructose-NAD and enzyme-NADH-sorbitol complexes.

对大鼠肝脏山梨醇脱氢酶（L-艾杜醇：NAD氧化还原酶，EC 1.1.1.14）的改良制剂进行了稳态动力学研究。2. 数据分析表明，该酶在山梨醇氧化方向遵循快速平衡随机机制，在果糖还原方向遵循随机机制。3. 动力学常数为：K m NAD 0.082 mM；K m山梨醇0.38 mM；K m NADH 67微摩尔；K m果糖136 mM。4. 有证据表明，较快的逆向（果糖还原）反应易受代谢控制，通过形成无效酶果糖-NAD和酶-NADH-山梨醇复合物实现。

Leissing N C, McGuinness E T

Int J Biochem. 1983;15(5):651-6. doi: 10.1016/0020-711x(83)90189-1.

A steady state kinetic investigation was performed on an improved preparation of rat-liver sorbitol dehydrogenase (L-iditol: NAD-oxidoreductase, EC 1.1.1.14). 2. Data analyses indicate the enzyme follows a rapid equilibrium random mechanism in the direction of sorbitol oxidation and a random mechanism in the direction of fructose reduction. 3. Kinetic constants were: K m NAD 0.082 mM; K m sorbitol 0.38 mM; K m NADH 67 microns; K m fructose 136 mM. 4. Evidence is adduced to indicate the more rapid reverse (fructose reduction) reaction is susceptible to metabolic control by formation of abortive enzyme fructose-NAD and enzyme-NADH-sorbitol complexes.

对大鼠肝脏山梨醇脱氢酶（L-艾杜醇：NAD氧化还原酶，EC 1.1.1.14）的改良制剂进行了稳态动力学研究。2. 数据分析表明，该酶在山梨醇氧化方向遵循快速平衡随机机制，在果糖还原方向遵循随机机制。3. 动力学常数为：K m NAD 0.082 mM；K m山梨醇0.38 mM；K m NADH 67微摩尔；K m果糖136 mM。4. 有证据表明，较快的逆向（果糖还原）反应易受代谢控制，通过形成无效酶果糖-NAD和酶-NADH-山梨醇复合物实现。