Naot Y, Guptill D R, Mullenax J, Remington J S
Infect Immun. 1983 Jul;41(1):331-8. doi: 10.1128/iai.41.1.331-338.1983.
Studies were performed to define the nature of the Toxoplasma gondii antigens that are recognized by human immunoglobulin M (IgM) and IgG antibodies. Both IgM and IgG antibodies were found to be directed mainly against T. gondii membrane antigens in sera obtained from patients with acute toxoplasmosis. Treatment of the membrane preparation with DNase, RNase, or lipase had no apparent effect on the reactivity of the membrane antigens with IgM and IgG antibodies. Lipids isolated from tachyzoites were not recognized by either IgM or IgG antibodies. Exposure of T. gondii membranes to heat, proteolysis, or oxidation with sodium periodate decreased the reactivity of the membrane preparations with both IgM and IgG antibodies. A preparation of T. gondii proteins and polysaccharides were recognized by both immunoglobulin classes. T. gondii polysaccharides reacted with human IgG antibodies produced during both the acute and chronic phases of the infection. We concluded that, after infection with T. gondii, IgM and IgG antibodies are elicited in response to both protein and carbohydrate constituents of the invading parasite.
开展了多项研究以确定被人类免疫球蛋白M（IgM）和IgG抗体识别的弓形虫抗原的性质。在急性弓形虫病患者的血清中发现，IgM和IgG抗体主要针对弓形虫膜抗原。用脱氧核糖核酸酶（DNase）、核糖核酸酶（RNase）或脂肪酶处理膜制剂，对膜抗原与IgM和IgG抗体的反应性没有明显影响。从速殖子中分离出的脂质未被IgM或IgG抗体识别。将弓形虫膜加热、进行蛋白水解或用过碘酸钠氧化，会降低膜制剂与IgM和IgG抗体的反应性。一类弓形虫蛋白质和多糖制剂可被这两种免疫球蛋白识别。弓形虫多糖与感染急性期和慢性期产生的人类IgG抗体发生反应。我们得出结论，感染弓形虫后，会针对入侵寄生虫的蛋白质和碳水化合物成分产生IgM和IgG抗体。
