在大肠杆菌中表达的重组弓形虫表面抗原1（P30）可被人弓形虫特异性免疫球蛋白M（IgM）和免疫球蛋白G（IgG）抗体识别。
Recombinant Toxoplasma gondii surface antigen 1 (P30) expressed in Escherichia coli is recognized by human Toxoplasma-specific immunoglobulin M (IgM) and IgG antibodies.
作者信息
Harning D, Spenter J, Metsis A, Vuust J, Petersen E
机构信息
Laboratory of Molecular Biology, Statens Seruminstitut, Copenhagen, Denmark.
出版信息
Clin Diagn Lab Immunol. 1996 May;3(3):355-7. doi: 10.1128/cdli.3.3.355-357.1996.
Abstract
The immunodominant surface antigen of Toxoplasma gondii, surface antigen 1 (SAG1), was expressed in Escherichia coli as a fusion protein containing a majority of the SAG1 protein supplied with six histidyl residues in the N-terminal end. The recombinant protein was purified on a Ni-chelate column and then on a fast-performance liquid chromatography column and was in a nonreduced condition. It was recognized by T. gondii-specific human immunoglobulin G (IgG) and IgM antibodies as well as by a mouse monoclonal antibody (S13) recognizing only nonreduced native SAG1. Antibodies induced in mice by the recombinant SAG1 recognized native SAG1 from the T. gondii RH isolate in culture. Recombinant SAG1 is suitable for use in diagnostic systems for detecting anti-SAG1-specific IgG and IgM antibodies.
摘要
刚地弓形虫的免疫显性表面抗原，即表面抗原1（SAG1），在大肠杆菌中作为融合蛋白表达，该融合蛋白包含大部分SAG1蛋白，其N端带有六个组氨酸残基。重组蛋白先在镍螯合柱上纯化，然后在快速液相色谱柱上纯化，且处于非还原状态。它能被刚地弓形虫特异性人免疫球蛋白G（IgG）和IgM抗体识别，也能被仅识别非还原天然SAG1的小鼠单克隆抗体（S13）识别。重组SAG1在小鼠体内诱导产生的抗体能识别培养的刚地弓形虫RH株的天然SAG1。重组SAG1适用于检测抗SAG1特异性IgG和IgM抗体的诊断系统。
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