McCoy J P, Schrier D, Lovett E J, Judd W J, Varani J
J Cell Sci. 1983 Jan;59:245-56. doi: 10.1242/jcs.59.1.245.
Commercially prepared ABO blood-typing antisera have been tested for their ability to bind to murine laminin and their cytotoxic effects upon high and low metastatic variants of a murine fibrosarcoma. Previous studies have shown that alpha-D-galactopyranosyl end-groups comprise the major antigenic determinants on type B erythrocytes and that these same end-groups are present on murine laminin purified from the EHS sarcoma and on a laminin-like glycoprotein on the surface of the high, but not low, metastatic fibrosarcoma cells. In the present study we found that all sera containing anti-B activity were cytotoxic to the high, but not the low, metastatic cells and that all of these sera reacted strongly against immobilized murine laminin in an enzyme-linked immunosorbent assay. Sera lacking anti-B activity, i.e. anti-A antisera, were much less cytotoxic to either cell line and three of the four anti-A sera did not bind to murine laminin. The laminin reactivity and cytotoxic effect of the anti-B sera were specifically abrogated by preincubation of the sera with water-soluble blood group B substance or with murine laminin but not with water-soluble blood group A substance.
已对市售的ABO血型定型抗血清与鼠层粘连蛋白的结合能力及其对鼠纤维肉瘤高转移和低转移变体的细胞毒性作用进行了测试。先前的研究表明，α-D-吡喃半乳糖基末端基团是B型红细胞上的主要抗原决定簇，并且这些相同的末端基团存在于从EHS肉瘤中纯化的鼠层粘连蛋白以及高转移而非低转移的纤维肉瘤细胞表面的一种层粘连蛋白样糖蛋白上。在本研究中，我们发现所有具有抗B活性的血清对高转移细胞具有细胞毒性，而对低转移细胞则无细胞毒性，并且所有这些血清在酶联免疫吸附测定中均与固定化的鼠层粘连蛋白发生强烈反应。缺乏抗B活性的血清，即抗A血清，对任一细胞系的细胞毒性都小得多，并且四种抗A血清中有三种不与鼠层粘连蛋白结合。通过将血清与水溶性B血型物质或鼠层粘连蛋白预孵育，而不与水溶性A血型物质预孵育，可特异性消除抗B血清的层粘连蛋白反应性和细胞毒性作用。
