高效液相色谱法结合荧光检测测定血浆中的α-酮酸。
High-performance liquid chromatographic determination of alpha-keto acids in plasma with fluorometric detection.
作者信息
Hayashi T, Tsuchiya H, Naruse H
出版信息
J Chromatogr. 1983 Apr 8;273(2):245-52. doi: 10.1016/s0378-4347(00)80946-9.
PMID:6863441
Abstract
This paper describes a sensitive high-performance liquid chromatographic method for the quantitative determination of alpha-keto acids in plasma using a fluorescence detector. This method is about ten times more sensitive than that reported in a previous paper. Only 50 microliters of plasma are needed for the determination of alpha-keto acids. However, p-hydroxyphenylpyruvic acid could not be analysed because the quinoxalinol derived from it does not exhibit fluorescence.
摘要
本文描述了一种使用荧光检测器定量测定血浆中α-酮酸的灵敏高效液相色谱法。该方法的灵敏度比之前一篇论文报道的方法高约十倍。测定α-酮酸仅需50微升血浆。然而，对羟基苯丙酮酸无法进行分析，因为由其衍生的喹喔啉醇不显示荧光。
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