大鼠肾上腺肿瘤细胞中[6-3H]-25-羟基胆固醇的合成与代谢
The synthesis and metabolism of [6-3H]-25-hydroxycholesterol in rat adrenal tumor cells.
作者信息
Mason J I, Murry B A, Aberhart D J
出版信息
J Steroid Biochem. 1983 Jun;18(6):765-9. doi: 10.1016/0022-4731(83)90257-1.
PMID:6865417
Abstract
The synthesis of [6-3H]-25-hydroxycholesterol from 26-norcholest-5-en-3 beta-ol-25-one is described. The metabolism of the radiolabeled hydroxysterol in rat adrenocortical carcinoma cells and in mitochondria-enriched preparations of rat adrenal tissue was investigated. We found that [6-3H]-25-hydroxycholesterol was metabolized efficiently to [3H]-pregnenolone in both preparations of rat adrenal tissue.
摘要
描述了从26-降胆甾-5-烯-3β-醇-25-酮合成[6-³H]-25-羟胆固醇的过程。研究了放射性标记的羟类固醇在大鼠肾上腺皮质癌细胞和大鼠肾上腺组织富含线粒体的制剂中的代谢情况。我们发现，在大鼠肾上腺组织的两种制剂中，[6-³H]-25-羟胆固醇都能有效地代谢为[³H]-孕烯醇酮。
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