Khansari N, Springer G F, Merler E, Fudenberg H H
Mech Ageing Dev. 1983 Jan;21(1):49-58. doi: 10.1016/0047-6374(83)90015-5.
Direct antiglobulin (Coombs') tests of erythrocyte (RBC) subpopulations confirmed the presence of membrane-bound immunoglobulin G (IgG) on old (density greater than 1.110) human RBCs but not on the young (density less than 1.110) RBCs. After thermal elution of the bound IgG, this Coombs' reaction was negative, but incubation of thermally eluted IgG (He-IgG) with heat-treated RBCs induced a positive antiglobulin test. A positive direct antiglobulin reaction was also obtained after incubation of heat-treated RBCs with anti-T antibody. Similar results were obtained when young RBCs treated with Vibrio cholerae neuraminidase (VCN) were incubated with anti-T or with IgG eluted by heat from old RBCs. Nevertheless, pre-absorption of heat-eluted IgG with T and/or Tn antigen, did not prevent it from binding to either heat-treated old or VCN-treated young RBCs as assessed by the antiglobulin consumption assay. Pre-treatment of either VCN-treated young or heat-treated old RBCs with anti-T and/or anti-Tn antibodies had no significant effect on the binding of radiolabeled He-IgG (eluted from old RBCs). The results indicate that even though desialylation of the erythrocyte membrane is required for binding of both anti-T-Tn and He-IgG, the specificity and consequently the RBC binding sites for He-IgG and anti-T seem to be different.
对红细胞（RBC）亚群进行的直接抗球蛋白（库姆斯氏）试验证实，在衰老的（密度大于1.110）人红细胞上存在膜结合免疫球蛋白G（IgG），而在年轻的（密度小于1.110）红细胞上则不存在。结合的IgG经热洗脱后，该库姆斯氏反应呈阴性，但热洗脱的IgG（He-IgG）与热处理的红细胞孵育会诱导抗球蛋白试验呈阳性。热处理的红细胞与抗-T抗体孵育后也获得了阳性直接抗球蛋白反应。当用霍乱弧菌神经氨酸酶（VCN）处理的年轻红细胞与抗-T或从衰老红细胞经热洗脱的IgG孵育时，也得到了类似的结果。然而，通过抗球蛋白消耗试验评估，用T和/或Tn抗原对热洗脱的IgG进行预吸收，并不能阻止其与热处理的衰老红细胞或VCN处理的年轻红细胞结合。用抗-T和/或抗-Tn抗体对VCN处理的年轻红细胞或热处理的衰老红细胞进行预处理，对放射性标记的He-IgG（从衰老红细胞洗脱）的结合没有显著影响。结果表明，尽管红细胞膜去唾液酸化是抗-T-Tn和He-IgG结合所必需的，但He-IgG和抗-T的特异性以及因此与红细胞的结合位点似乎是不同的。
