Holt J A, Lorincz M A, King W J
Obstet Gynecol. 1983 Aug;62(2):231-5.
Monoclonal antibody against human breast cancer estrogen receptor was used to demonstrate binding of a gamma- and Auger electron-emitting estrogen to the estrogen receptor in ovarian epithelial carcinomas. When cytosols of estrogen receptor-rich ovarian adenocarcinomas were analyzed on sucrose gradients containing 0.4 M KCl, the presence of monoclonal antibody against breast cancer estrogen receptor caused a binding peak for [125I]- and [3H]estradiol to shift from the 4S to the 8-9S region, indicating antibody complex formation with ovarian adenocarcinoma estrogen receptor. The antibody-shifted peak of 8-9S [125I]- and [3H]estradiol binding was totally inhibited by a 100-fold molar excess of diethylstilbestrol (DES), but not by testosterone or progesterone, indicating a preference for estrogen binding by the antibody-shifted estrogen receptor. When estrogen receptor from the nuclear fraction of ovarian adenocarcinomas was incubated with [125I]estradiol at 1C, in the presence of 0.4 M NaSCN to facilitate exchange with endogenous ligand, binding occurred that was inhibitable by DES and restricted to the 4S region. Under these conditions the nuclear estrogen receptor was also shifted to the 8-9S region by the presence of the monoclonal antibody against estrogen receptor.
使用抗人乳腺癌雌激素受体的单克隆抗体来证明一种发射γ射线和俄歇电子的雌激素与卵巢上皮癌中雌激素受体的结合。当在含有0.4M氯化钾的蔗糖梯度上分析富含雌激素受体的卵巢腺癌的胞质溶胶时，抗乳腺癌雌激素受体单克隆抗体的存在导致[125I] - 和[3H]雌二醇的结合峰从4S转移到8 - 9S区域，表明与卵巢腺癌雌激素受体形成抗体复合物。8 - 9S [125I] - 和[3H]雌二醇结合的抗体转移峰被100倍摩尔过量的己烯雌酚（DES）完全抑制，但不被睾酮或孕酮抑制，表明抗体转移的雌激素受体对雌激素结合具有偏好性。当卵巢腺癌核部分的雌激素受体在1℃下与[125I]雌二醇一起孵育时，在存在0.4M硫氰酸钠以促进与内源性配体交换的情况下，发生了可被DES抑制且局限于4S区域的结合。在这些条件下，抗雌激素受体单克隆抗体的存在也使核雌激素受体转移到8 - 9S区域。
