Saito R, Kawasaki K, Ono N, Kamiya H
Res Commun Chem Pathol Pharmacol. 1983 Apr;40(1):41-53.
The effect of imipramine on spontaneous efflux of radiolabelled dopamine (DA) from slices of rat striatum was examined by a superfusion method. Imipramine at concentrations of 10 - 100 microM enhanced the efflux of DA accumulated in a high-affinity uptake system in a concentration-dependent manner. This efflux of 3H-DA was not affected by conditions (Ca2+-free medium, 100 microM bretylium and 30 microM tetrodotoxin) which inhibited the release of 3H-DA by electrical stimulation. Furthermore, this imipramine-induced 3H-DA efflux was temperature-dependent. The uptake of 3H-imipramine into striatal slices was determined. This uptake was concentration- and temperature-dependent and increased linearly. These results are discussed in relation to the hypothesis that 3H-DA efflux by imipramine is connected with uptake of imipramine.
采用灌流法研究了丙咪嗪对大鼠纹状体切片中放射性标记多巴胺（DA）自发外流的影响。浓度为10 - 100微摩尔的丙咪嗪以浓度依赖的方式增强了在高亲和力摄取系统中积累的DA的外流。3H-DA的这种外流不受抑制电刺激释放3H-DA的条件（无钙培养基、100微摩尔溴苄铵和30微摩尔河豚毒素）的影响。此外，丙咪嗪诱导的3H-DA外流是温度依赖性的。测定了3H-丙咪嗪进入纹状体切片的摄取情况。这种摄取是浓度和温度依赖性的，并且呈线性增加。结合丙咪嗪引起的3H-DA外流与丙咪嗪摄取有关的假说对这些结果进行了讨论。
