Siddiqui A H, Janssen de Limpens A M, Cormane R H
Arch Dermatol Res. 1978 Jul 28;262(2):229-33. doi: 10.1007/BF00455394.
A simplified method is described to demonstrate Y chromosome fluorescence in interphase nuclei of epithelial cells in human buccal smears using Quinacrine Mustard. This technique turned out to be efficient in our hands and would be of value in determining the sex chromosomes in the epithelial cells of the skin in cases of allogeneic transplantations from females to males and vice versa to determine the peroid during which the grafts survived.
本文描述了一种简化方法,用于使用喹吖因氮芥在人类口腔涂片上皮细胞的间期核中显示Y染色体荧光。在我们的实践中,该技术证明是有效的,并且在同种异体女性向男性或男性向女性移植的情况下,对于确定皮肤上皮细胞中的性染色体以及确定移植物存活的时期具有价值。