一种改良的组织蛋白酶-D底物及检测方法。

An improved cathepsin-D substrate and assay procedure.

作者信息

Agarwal N, Rich D H

出版信息

Anal Biochem. 1983 Apr 1;130(1):158-65. doi: 10.1016/0003-2697(83)90663-2.

Abstract

Ten analogs of the peptide A-Phe(NO2)-Phe-Val-Leu-B were synthesized and tested as substrates for cathepsin D and pepsin. The best substrate found for cathepsin D, Phe-Ala-Ala-Phe(NO2)-Phe-Val-Leu-OM4P (kcat = 2.9 s-1; Km = 7.1 microM), has the largest kcat/Km value (408 mM-1 s-1) reported to date for this enzyme. The effect of peptide structure on solubility and kinetic parameters is discussed. The peptide provides a useful new substrate for continuous assay of cathepsin D.

摘要

合成了肽A-Phe(NO2)-Phe-Val-Leu-B的十种类似物，并将其作为组织蛋白酶D和胃蛋白酶的底物进行测试。发现组织蛋白酶D的最佳底物Phe-Ala-Ala-Phe(NO2)-Phe-Val-Leu-OM4P（kcat = 2.9 s-1；Km = 7.1 microM），其kcat/Km值（408 mM-1 s-1）是迄今为止报道的该酶的最大值。讨论了肽结构对溶解度和动力学参数的影响。该肽为组织蛋白酶D的连续测定提供了一种有用的新底物。