一种从马铃薯块茎中纯化金属羧肽酶抑制剂的快速、大规模方法。

A rapid, large-scale method for purification of the metallo-carboxypeptidase inhibitor from potato tubers.

作者信息

Pearce G, Ryan C A

出版信息

Anal Biochem. 1983 Apr 1;130(1):223-5. doi: 10.1016/0003-2697(83)90673-5.

Abstract

A rapid, large-scale procedure has been developed for the preparation of metallo-carboxypeptidase inhibitor (CPI) from potatoes. The inhibitor is extracted with 80% ethanol from partially cooked potato tubers. After the ethanol is removed and the salts are dialyzed away, the inhibitor is isolated in pure form by chromatography on sulfopropyl Sephadex. Ten pounds of potatoes yields 250 mg pure inhibitor, representing over 40% recovery. Commercial mashed potato flakes contain about 0.02% carboxypeptidase inhibitor and can be utilized as a starting material in the procedure described to yield over 70 mg inhibitor from a pound of flakes.

摘要

已开发出一种快速、大规模从土豆中制备金属羧肽酶抑制剂（CPI）的方法。用80%乙醇从部分煮熟的土豆块茎中提取该抑制剂。去除乙醇并透析除去盐分后，通过磺丙基葡聚糖凝胶柱色谱法以纯形式分离该抑制剂。十磅土豆可产出250毫克纯抑制剂，回收率超过40%。市售土豆泥薄片含有约0.02%的羧肽酶抑制剂，可作为所述方法的起始原料，一磅薄片可产出超过70毫克抑制剂。