Drewa G, Zbytniewski Z, Kanclerz A
Arch Geschwulstforsch. 1978;48(3):198-201.
Activity of arylsulphatase, beta-glucuronidase, cathepsin D, and acid phosphatase in the homogenates of melanotic and amelanotic melanoma was determined. The activity of these enzymes is higher in melanotic than in amelanotic melanoma. Respective values for melanotic and amelanotic tumours are: arylsulphatase 10,78 +/- 3,20, and 1,45 +/- 0,66 micron 4-nitrocatechole/mg protein/hr; beta-glucuronidase 11,10 +/- 1,40, and 9,98 +/- 1,35 micron phenolphthalein/mg protein/hr; cathepsin D 4,24 +/- 1,37, and 3,26 +/- 0,73 micron tyrosine/mg protein/hr; acid phosphatase 230 +/- 22, and 180 +/- 25 micron p-nitrophenol/mg protein/hr. These differences are statistically significant. The increased activity of the lysosomal enzymes in melantoic melanoma probably depends on the occurrence of an higher number of lysosomes in tissues containing melanins.
测定了黑色素瘤和无黑色素瘤匀浆中芳基硫酸酯酶、β-葡萄糖醛酸酶、组织蛋白酶D和酸性磷酸酶的活性。这些酶在黑色素瘤中的活性高于无黑色素瘤。黑色素瘤和无黑色素瘤肿瘤的相应值分别为：芳基硫酸酯酶10.78±3.20和1.45±0.66微摩尔4-硝基邻苯二酚/毫克蛋白/小时；β-葡萄糖醛酸酶11.10±1.40和9.98±1.35微摩尔酚酞/毫克蛋白/小时；组织蛋白酶D 4.24±1.37和3.26±0.73微摩尔酪氨酸/毫克蛋白/小时；酸性磷酸酶230±22和180±25微摩尔对硝基苯酚/毫克蛋白/小时。这些差异具有统计学意义。黑色素瘤中溶酶体酶活性的增加可能取决于含黑色素组织中溶酶体数量的增加。
