从牛羊水分离出queuine。
Isolation of queuine from bovine amniotic fluid.
作者信息
Reyniers J P, Farkas W R
出版信息
Anal Biochem. 1983 Apr 15;130(2):427-30. doi: 10.1016/0003-2697(83)90611-5.
PMID:6869830
Abstract
A facile method for isolation of large quantities of queuine from bovine amniotic fluid is described. Queuine was sequentially purified by cation-exchange chromatography, adsorption chromatography on Sephadex G-25, and size-exclusion chromatography on Sephadex G-10. The queuine isolate was identified by its participation in the queuine-guanine tRNA transglycosylase reaction and comparisons with authentic queuine.
摘要
本文描述了一种从牛羊水分离大量queuine的简便方法。通过阳离子交换色谱、Sephadex G - 25吸附色谱和Sephadex G - 10尺寸排阻色谱依次纯化queuine。通过其参与queuine - 鸟嘌呤tRNA转糖基酶反应并与纯queuine比较来鉴定所分离的queuine。
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