Boothby J T, Jasper D E, Zinkl J G, Thomas C B, Dellinger J D
Am J Vet Res. 1983 May;44(5):831-8.
Microbiological cultural, cytologic, and immunologic observations were made on 30 calves. The eyes, nares, and bronchioalveolar region were subjected to microbiological cultural examination for mycoplasmas. Four of the examinations of 30 eyes, 15 of those of 30 nasal tissues, and 25 of those of the 30 bronchioalveolar regions from the 30 calves were positive for mycoplasmas. Mycoplasma bovis and M bovirhinis were the most prevalent species. Cytologic examinations of peripheral blood and bronchioalveolar washes did not show pathologic changes. Results of indirect hemagglutination, enzyme-linked immunosorbent assay, lymphocyte-stimulation tests on peripheral blood cells, and skin testing demonstrated only a low prevalence of immune recognition of M bovis. Infection and immune response were studied in 3 calves for 10 weeks before, and for 4 weeks after, intratracheal administration of live M bovis.
对30头犊牛进行了微生物培养、细胞学和免疫学观察。对眼睛、鼻孔和支气管肺泡区域进行支原体微生物培养检查。30头犊牛的30只眼睛检查中有4只、30个鼻组织检查中有15只、30个支气管肺泡区域检查中有25个支原体检测呈阳性。牛支原体和牛鼻支原体是最常见的种类。外周血和支气管肺泡灌洗的细胞学检查未显示病理变化。间接血凝试验、酶联免疫吸附测定、外周血细胞淋巴细胞刺激试验和皮肤试验结果表明，对牛支原体的免疫识别率仅较低。在3头犊牛气管内接种活牛支原体之前10周和之后4周研究感染和免疫反应。
