Pieroni G, Hanahan D J
Arch Biochem Biophys. 1983 Jul 15;224(2):485-93. doi: 10.1016/0003-9861(83)90236-9.
The metabolic fate of 1-O-[3H]alkyl-2-acetyl-sn-glycero-3-phosphorylcholine ([3H]-AGEPC) upon interaction with rabbit platelets was investigated. [3H]AGEPC was converted to a product identified as the long-chain fatty acyl analog. The reaction was unaffected by extracellular calcium. After a lag time of 30 to 60 s the kinetics of the conversion was linear. The rate of the reaction was found to be a function of platelet and AGEPC concentrations. Of the [3H]AGEPC (10(-9) M) 85 +/- 5% was processed into the-long chain fatty acyl analog within 1 h when incubated at 37 degrees C with a 1.25 X 10(9) platelets per milliliter suspension. A maximal number of 1200 to 3600 [3H]AGEPC molecules were converted to the long-chain fatty acyl derivative per minute per platelet in the presence of 2 mM EDTA. Under similar conditions the 1-O-[3H]alkyl-2-(lyso)-sn-glycero-3-phosphorylcholine ([3H]lysoGEPC) also was transformed to a comparable long-chain fatty acyl derivative at a much slower rate and to a lower extent. No significant increase in lysoGEPC was noted in incubation mixtures containing [3H]AGEPC. The possible direct transacylation of AGEPC upon interaction with platelets is discussed as well as the possible involvement of this reaction in directly triggering the platelet response to AGEPC stimuli.
研究了1-O-[³H]烷基-2-乙酰基-sn-甘油-3-磷酸胆碱（[³H]-AGEPC）与兔血小板相互作用后的代谢命运。[³H]AGEPC转化为一种被鉴定为长链脂肪酰类似物的产物。该反应不受细胞外钙的影响。经过30至60秒的延迟时间后，转化动力学呈线性。发现反应速率是血小板和AGEPC浓度的函数。当在37℃下与每毫升悬浮液1.25×10⁹个血小板一起孵育时，在1小时内，85±5%的[³H]AGEPC（10⁻⁹M）被加工成长链脂肪酰类似物。在2mM EDTA存在下，每个血小板每分钟最多有1200至3600个[³H]AGEPC分子转化为长链脂肪酰衍生物。在类似条件下，1-O-[³H]烷基-2-（溶血）-sn-甘油-3-磷酸胆碱（[³H]溶血GEPC）也以慢得多的速率和较低的程度转化为类似的长链脂肪酰衍生物。在含有[³H]AGEPC的孵育混合物中未观察到溶血GEPC有显著增加。讨论了AGEPC与血小板相互作用时可能的直接转酰基作用，以及该反应可能在直接触发血小板对AGEPC刺激的反应中的作用。
