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三趾树懒室管膜表面的相关扫描和透射电子显微镜研究

Correlative scanning and transmission electron microscopic study on the ependymal surface of Bradypus tridactylus.

作者信息

Ferraz de Carvalho C A, Chagas C F, da Silva C F, Prado Reis F

出版信息

Arq Neuropsiquiatr. 1983 Mar;41(1):64-72. doi: 10.1590/s0004-282x1983000100005.

DOI:10.1590/s0004-282x1983000100005
PMID:6870587
Abstract

Topographical variations of the ependymal surface of the whole brain ventricular system of Bradypus tridactylus were studied at the scanning electron microscope after CO2 critical point drying and carbon-gold coating. Certain selected areas were also studied at the transmission electron microscope, following standard technical procedures. We observed distinct patterns in the distribution of cilia, microvilli and supraependymal structures (nerve-like and fibrous astrocyte fibers), and also a small number of blebs. It the lateral transition zone between the floor and roof of the lateral ventricle were found interconnected stellate bulgings measuring an average 98 x 190 microns, constituted by a complex meshwork of processes from fibrous astrocytes, without ependymal lining and with few cell bodies. In the junction between these formations and the underlying neuropil, ciliated ependymal-cell-like glial cells were found to delimit complex labyrinthic spaces. Based on the morphological findings, morpho-functional considerations are made.

摘要

在二氧化碳临界点干燥和碳金涂层后,利用扫描电子显微镜研究了三趾树懒全脑室系统室管膜表面的地形变化。按照标准技术程序,还对某些选定区域进行了透射电子显微镜研究。我们观察到纤毛、微绒毛和室管膜上结构(神经样和纤维性星形胶质细胞纤维)分布的明显模式,以及少量泡状结构。在侧脑室底部和顶部之间的外侧过渡区发现了相互连接且平均尺寸为98×190微米的星状凸起,由纤维性星形胶质细胞的复杂突起网络构成,无室管膜衬里且细胞体较少。在这些结构与下方神经毡的交界处,发现有纤毛的室管膜细胞样胶质细胞界定了复杂的迷宫状空间。基于形态学发现,进行了形态功能方面的思考。

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