破伤风毒素重链和轻链在氨基酸组成上表现出的相似性。
Similarities in the heavy and light chains of tetanus toxin suggested by their amino acid compositions.
作者信息
Taylor C F, Britton P, van Heyningen S
出版信息
Biochem J. 1983 Mar 1;209(3):897-9. doi: 10.1042/bj2090897.
Abstract
Quantitative comparison of the amino acid compositions of the heavy and light chains of tetanus toxin by the method of Cornish-Bowden [(1983) Methods Enzymol. 91, 60-75)] suggests strongly that there is sequence homology between the two chains and that the heavy chain has two similar halves. Examination (by electrophoresis in polyacrylamide gels in the presence of sodium dodecyl sulphate) of peptides produced from the chains by proteolytic cleavage supports this idea.
摘要
采用康沃尔-鲍登方法[(1983)《酶学方法》91卷，60 - 75页]对破伤风毒素重链和轻链的氨基酸组成进行定量比较，结果有力地表明两条链之间存在序列同源性，且重链有两个相似的部分。通过（在十二烷基硫酸钠存在下于聚丙烯酰胺凝胶中进行电泳）对经蛋白水解切割从链上产生的肽段进行检测，支持了这一观点。
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