Prasad M R, Nagi M N, Cook L, Cinti D L
Biochem Biophys Res Commun. 1983 Jun 15;113(2):659-65. doi: 10.1016/0006-291x(83)91777-1.
The rat hepatic microsomal conversion of crotonyl- and hexenoyl CoA to butyrate and hexanoate was supported only by NADPH, while both NADH and NADPH were effective cofactors in the conversion of trans-2-hexadecenoyl CoA to palmitate. Experiments using mixtures of long- and short-chain enoyl-CoA substrates and competition experiments support the conclusion that microsomes contain 2 distinct enoyl CoA reductases, (1) a long chain enoyl CoA reductase capable of accepting reducing equivalents from either NADH or NADPH, and (2) a NADPH-specific short chain enoyl CoA reductase.
仅NADPH可支持大鼠肝微粒体将巴豆酰辅酶A和己烯酰辅酶A转化为丁酸和己酸，而在反式-2-十六碳烯酰辅酶A转化为棕榈酸的过程中，NADH和NADPH都是有效的辅助因子。使用长链和短链烯酰辅酶A底物混合物的实验以及竞争实验支持以下结论：微粒体含有2种不同的烯酰辅酶A还原酶，（1）一种长链烯酰辅酶A还原酶，能够接受来自NADH或NADPH的还原当量，（2）一种NADPH特异性短链烯酰辅酶A还原酶。
