Kosciuszko H, Koizumi S
Exp Cell Res. 1983 Jul;146(2):436-8. doi: 10.1016/0014-4827(83)90146-5.
Macronuclear karyoplasm was transplanted from pre-autogamous donor cells (clonal age, 22 fissions) into the macronucleus of young recipient cells (2 fissions after autogamy occurred) by means of microinjection. A reciprocal experiment was carried out by injecting karyoplasm from young clonal age donors into pre-autogamous recipients. In the case of karyoplasm transfer from pre-autogamous donors to young recipients, autogamy occurred early in 67% of injected cells, whereas reciprocal injections had no influence on the onset of autogamy, and all of the injected cells underwent autogamy. Such results indicate a distinct role of pre-autogamous cells of macronucleus in the induction of autogamy.
通过显微注射的方式，将来自自体受精前供体细胞（克隆龄为22次分裂）的大核核质移植到年轻受体细胞（自体受精发生后2次分裂）的大核中。通过将来自年轻克隆龄供体的核质注射到自体受精前受体中进行了反向实验。在从自体受精前供体向年轻受体转移核质的情况下，67%的注射细胞中自体受精提前发生，而反向注射对自体受精的开始没有影响，所有注射细胞都进行了自体受精。这些结果表明大核的自体受精前细胞在诱导自体受精中具有独特作用。
