Sato A, Shimada K, Izumo Y, Sakaguchi T
J Chromatogr. 1983 Jun 10;275(1):97-105. doi: 10.1016/s0378-4347(00)84348-0.
Simple and rapid quantitative analysis utilizing high-performance liquid chromatography was performed to determine the concentration of the antiepileptic agent phenytoin, and its main metabolite hydroxyphenytoin, in human urine. For the purposes of simple and rapid determination, the commercially available Extrelut columns were used with diethyl ether and chloroform as extraction solvents. High-performance liquid chromatography was performed on a LiChrosorb RP-8 column, with a mobile phase of methanol-0.02% ammonium acetate (1:1). The internal standard was 5-(4-methylphenyl)-5-phenylhydantoin. The method was applied to patients' urine, to examine the influence of concomitant drugs. Also, the results obtained using the commercially available enzymatic immunoassay method were compared with those from the present method, and it was concluded that a simple and rapid microanalysis is possible with a high extraction ratio.
采用高效液相色谱法进行简单快速的定量分析，以测定人尿中抗癫痫药物苯妥英及其主要代谢物羟基苯妥英的浓度。为实现简单快速测定，使用市售的Extrelut柱，以乙醚和氯仿作为萃取溶剂。在LiChrosorb RP - 8柱上进行高效液相色谱分析，流动相为甲醇 - 0.02%醋酸铵（1:1）。内标为5 -（4 - 甲基苯基）- 5 - 苯基乙内酰脲。该方法应用于患者尿液，以检测合并用药的影响。此外，将使用市售酶免疫分析法获得的结果与本方法的结果进行比较，得出结论：该方法萃取率高，可实现简单快速的微量分析。
