Yamada Y
J Biochem Biophys Methods. 1983 May;7(3):175-85. doi: 10.1016/0165-022x(83)90027-1.
A new apparatus has been developed to facilitate a good, effective transfer of protein from a first-dimension gel into a second-dimension gel. This new simple apparatus makes running time of the slab gel short and is convenient for the simultaneous processing of many polyacrylamide gel slabs and ultra-thin (100 micrometers) gels. Many gels can be run at the same time in a small space and be readily inserted or removed. Multiple gels can easily be prepared. The plates can have any desired shape and dimension, and their thickness can easily be changed. The present method is very simple and inexpensive, has a wide application and has many other advantages.
已开发出一种新装置，以促进蛋白质从一维凝胶到二维凝胶的良好、有效转移。这种新的简单装置缩短了平板凝胶的运行时间，便于同时处理多个聚丙烯酰胺凝胶平板和超薄（100微米）凝胶。许多凝胶可以在小空间内同时运行，并且易于插入或取出。可以轻松制备多个凝胶。平板可以具有任何所需的形状和尺寸，并且其厚度可以轻松改变。本方法非常简单且成本低廉，具有广泛的应用且还有许多其他优点。
