Lackie J M, Burns M D
J Immunol Methods. 1983 Aug 12;62(1):109-22. doi: 10.1016/0022-1759(83)90116-3.
A semi-automated method, using a graphic digitizing tablet, for analysing the the tracks of cells taken from time-lapse film is described and illustrated with sample data. Three film sequences of neutrophil leucocytes moving on protein-coated substrata are analysed, and in one of these the cells are moving in a non-random fashion. The randomness of the tracks is tested by an objective method. Cells in the non-random (directed) sequence move faster and make smaller angled turns. No correlation between step length and turning angle could be shown for the random sequences. The method described here should make it possible to undertake visual analyses of cell locomotion with considerably less effort.
本文描述了一种使用图形数字化平板电脑的半自动方法，用于分析从延时电影中获取的细胞轨迹，并给出了示例数据进行说明。分析了三个中性粒细胞在蛋白质包被基质上移动的电影序列，其中一个序列中的细胞以非随机方式移动。通过一种客观方法测试轨迹的随机性。非随机（定向）序列中的细胞移动速度更快，转弯角度更小。对于随机序列，步长和转弯角度之间没有相关性。这里描述的方法应该能够以少得多的工作量对细胞运动进行可视化分析。
