Vasan N
J Embryol Exp Morphol. 1983 Feb;73:263-74.
Notochordal proteoglycans influence somite chondrogenesis. In earlier work we learned that notochord cultured under improved conditions for 8 days contains three different classes of perinotochordal proteoglycan molecules, are necessary for inducing somite chondrogenesis. When the notochords were exposed to substances causing metabolic alterations (BUdR, excess vitamin A, and hyaluronic acid), only small proteoglycans accumulated in the perinotochordal sheath. Such treated notochords or the proteoglycans extracted from them failed to support somite chondrogenesis, suggesting that the large aggregate in the perinotochordal matrix may be necessary for somite induction. Adding a crude proteoglycan prepared from embryonic sternal cartilage increased the induction of chondrogenesis in somites, but papain treatment of the proteoglycan diminished its inductive capacity. This result further suggests that large aggregate proteoglycan may be necessary for the tissue interactions that promote chondrogenesis.
脊索蛋白聚糖影响体节软骨形成。在早期的研究中我们了解到，在改良条件下培养8天的脊索含有三类不同的围脊索蛋白聚糖分子，它们是诱导体节软骨形成所必需的。当脊索暴露于引起代谢改变的物质（5-溴脱氧尿苷、过量维生素A和透明质酸）时，只有小蛋白聚糖积聚在围脊索鞘中。经过这种处理的脊索或从中提取的蛋白聚糖无法支持体节软骨形成，这表明围脊索基质中的大聚集体可能是体节诱导所必需的。添加从胚胎胸骨软骨制备的粗蛋白聚糖可增加体节软骨形成的诱导，但用木瓜蛋白酶处理该蛋白聚糖会降低其诱导能力。这一结果进一步表明，大聚集体蛋白聚糖对于促进软骨形成的组织相互作用可能是必需的。
