Patton S E, O'Callaghan J P, Miller D B, Abou-Donia M B
J Neurochem. 1983 Sep;41(3):897-901. doi: 10.1111/j.1471-4159.1983.tb04826.x.
The effects of a single oral dose of 750 mg/kg tri-o-cresyl phosphate (TOCP) on the endogenous phosphorylation of specific brain proteins were assessed in male adult chickens following the development of delayed neurotoxicity. Phosphorylation of crude synaptosomal (P2) membrane and synaptosomal cytosolic proteins was assayed in vitro by using [gamma-32P]ATP as phosphate donor. Following resolution of brain proteins by sodium dodecyl sulfate polyacrylamide gel electrophoresis, specific protein phosphorylation was detected by autoradiography and quantified by microdensitometry. TOCP administration enhanced the phosphorylation of both cytosolic (Mr 65,000 and 55,000) and membrane (20,000) proteins by as much as 146% and 200%, respectively.
在雄性成年鸡出现迟发性神经毒性后，评估单次口服剂量为750毫克/千克的磷酸三邻甲苯酯（TOCP）对特定脑蛋白内源性磷酸化的影响。通过使用[γ-32P]ATP作为磷酸盐供体，在体外测定粗突触体（P2）膜和突触体胞质蛋白的磷酸化。通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳分离脑蛋白后，通过放射自显影检测特定蛋白的磷酸化，并通过显微密度测定法定量。给予TOCP后，胞质蛋白（分子量65,000和55,000）和膜蛋白（分子量20,000）的磷酸化分别增加了146%和200%。
