De Mey J G, Vanhoutte P M
J Physiol. 1983 Feb;335:65-74. doi: 10.1113/jphysiol.1983.sp014519.
Experiments were designed to determine the role of the endothelium in the responsiveness of the arterial wall to anoxia. Paired rings of canine femoral arteries were mounted for isometric tension recording in organ chambers filled with aerated Krebs-Ringer bicarbonate solution (37 degrees C). One ring served as control; in the other the intimal layer was removed mechanically. Anoxia was induced by gassing the organ chamber with 95% N2/5% CO2. In control rings anoxia augmented contractile responses to noradrenaline, KCl and BaCl2. On return to O2 the contractile responses were transiently depressed. Removal of the endothelium reduced the anoxic augmentation, but did not affect the post-anoxic inhibition. Indomethacin did not affect the response to anoxia. Anoxia abolished the endothelium-dependent inhibitory effect of acetylcholine and thrombin, reduced that of adenosine triphosphate, but augmented that of arachidonic acid. These experiments indicate that endothelial cells may contribute to anoxic facilitation of the responsiveness of the canine arterial wall.
实验旨在确定内皮在动脉壁对缺氧反应性中的作用。将成对的犬股动脉环安装在充满通气的 Krebs-Ringer 碳酸氢盐溶液（37 摄氏度）的器官浴槽中，用于等长张力记录。一个环作为对照；另一个环的内膜层通过机械方法去除。通过用 95% N2/5% CO2 给器官浴槽充气来诱导缺氧。在对照环中，缺氧增强了对去甲肾上腺素、氯化钾和氯化钡的收缩反应。恢复到氧气环境时，收缩反应短暂降低。去除内皮减少了缺氧增强作用，但不影响缺氧后抑制。吲哚美辛不影响对缺氧的反应。缺氧消除了乙酰胆碱和凝血酶的内皮依赖性抑制作用，降低了三磷酸腺苷的抑制作用，但增强了花生四烯酸的抑制作用。这些实验表明，内皮细胞可能有助于犬动脉壁对缺氧反应性的促进。
