肾小管周蛋白对体外灌注的兔近端曲管中液体重吸收的影响。

The effect of peritubular protein upon fluid reabsorption in rabbit proximal convoluted tubules perfused in vitro.

作者信息

Pirie S C, Potts D J

出版信息

J Physiol. 1983 Apr;337:429-40. doi: 10.1113/jphysiol.1983.sp014633.

DOI:10.1113/jphysiol.1983.sp014633

PMID:6875940

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1199116/

Abstract

This paper describes an investigation of the effects of varying the peritubular protein concentration upon the rate of fluid reabsorption of proximal convoluted tubule segments of the rabbit kidney, isolated and perfused in vitro. Eleven moderately distended tubules, bathed with rabbit serum (60 g l.-1 protein) and perfused with an ultrafiltrate of serum (ca. 0 g l.-1) protein, reabsorbed fluid at a rate of 1.0 +/- 0.07 nl. mm-1 min-1. When the protein of the bathing solution was reduced by replacing the serum with ultrafiltrate there was little change in fluid reabsorption rate. A further eleven moderately distended tubules, bathed with physiological saline containing bovine serum albumin (60 g l.-1) and perfused with saline, reabsorbed fluid at 1.0 +/- 0.06 nl. mm-1 min-1. There was little change in fluid reabsorption when the protein concentration was reduced to either 20 or 0 g l.-1; these tubules responded to 10(-5) M-ouabain in the bath, or a temperature of 25 degrees C, by reducing their absorption rate to 0.13 +/- 0.05 nl. mm-1 min-1. Nine minimally distended tubules reabsorbed fluid at 0.8 nl. mm-1 min-1 when bathed with physiological saline containing bovine serum albumin (60 g l.-1) and perfused with saline. Under these circumstances reabsorption rate fell by 26% when protein was removed from the bath. A study of the pressure-diameter relationship was made for eight tubules typical of those used in this laboratory. The probable range of lumen hydrostatic pressures was discussed for the distended and undistended states. We conclude that the effect of peritubular protein concentration upon fluid reabsorption in proximal convoluted tubule segments perfused in vitro is dependent upon some property of the tubule wall that is changed when distension occurs.

摘要

本文描述了一项关于改变肾小管周围蛋白质浓度对兔肾近端曲管节段液体重吸收速率影响的研究，这些节段在体外进行分离和灌注。11个适度扩张的肾小管，用兔血清（蛋白质浓度60 g·l⁻¹）进行浴灌，并灌注血清超滤液（蛋白质浓度约为0 g·l⁻¹），液体重吸收速率为1.0±0.07 nl·mm⁻¹·min⁻¹。当用超滤液替代血清使浴灌溶液中的蛋白质减少时，液体重吸收速率几乎没有变化。另外11个适度扩张的肾小管，用含牛血清白蛋白（60 g·l⁻¹）的生理盐水进行浴灌，并灌注生理盐水，液体重吸收速率为1.0±0.06 nl·mm⁻¹·min⁻¹。当蛋白质浓度降至20 g·l⁻¹或0 g·l⁻¹时，液体重吸收几乎没有变化；这些肾小管在浴灌中加入10⁻⁵ M哇巴因或温度为25℃时，其吸收速率降至0.13±0.05 nl·mm⁻¹·min⁻¹。9个轻微扩张的肾小管在用含牛血清白蛋白（60 g·l⁻¹）的生理盐水进行浴灌并灌注生理盐水时，液体重吸收速率为0.8 nl·mm⁻¹·min⁻¹。在这种情况下，当从浴灌中去除蛋白质时，重吸收速率下降了26%。对本实验室使用的8个典型肾小管进行了压力 - 直径关系的研究。讨论了扩张和未扩张状态下管腔静水压力的可能范围。我们得出结论，体外灌注的近端曲管节段中，肾小管周围蛋白质浓度对液体重吸收的影响取决于肾小管壁的某些特性，这些特性在发生扩张时会发生变化。