Interactions between photosystem II components in chloroplast membranes. A correlation between the existence of a low potential species of cytochrome b-559 and low chlorophyll fluorescence in inhibited and developing chloroplasts.

1. Chloroplasts inhibited by incubation with hydroxylamine in the light exhibit a low fluorescence yield upon illumination in the presence of dithionite sufficient to completely reduce the primary acceptor, Q. In the absence of magnesium ions, the fluorescence yield is the same as in control chloroplasts, suggesting that the reason for the low yield is a defect in the mechanism by which Mg2+ enhances the fluorescence. These chloroplasts were previouly shown to contain only low potential (Em7.8 = +80 mV) cytochrome b-559 (Horton, P. and Croze, E (1977) Biochim. Biophys. Acta 462, 86-101). 2. In Photosystem II particles, in heat-treated chloroplasts and in trypsin-digested chloroplasts, high potential cytochrome b-559 is absent and the variable fluorescence yield is again low. 3. Peas grown under intermittent light contain only one-fifth of the content of high potential cytochrome b-559 seen in fully greened plants, yet show high rates of water to methyl viologen electron transport. Aquisition of the high potential cytochrome b-559 accompanies synthesis of chlorophyll b, the onset of Mg-stimulated fluorescence and an increased variable yield of fluorescence. A similar correlation was seen during greening of dark-grown barley. 4. It is proposed that the high potential state of cytochrome b-559 is due to the same membrane properties which allow cation enhanced variable fluorescence, so that the presence of low potential cytochrome b-559 is accompanied by a decrease in variable fluorescence yield.