阿霉素（多柔比星）和α-生育酚（维生素E）对多形核白细胞功能的调节作用

Modulation of polymorphonuclear leukocyte function by doxorubicin (Adriamycin) and alpha tocopherol (vitamin E).

作者信息

Pickering L K, Cleary T G, Kletzel M, Wang Y M, Parra M M

出版信息

J Clin Lab Immunol. 1983 Jun;11(2):95-100.

Abstract

The effect of various concentrations of Adriamycin alone and Adriamycin plus vitamin E on human polymorphonuclear leukocyte (PMNL) function was determined. Aliquots of PMNLs were incubated at 37 degrees C with saline or vitamin E and then exposed to Adriamycin. A significant decrease in 1-14C glucose utilization and oxygen consumption by zymosan stimulated PMNLs occurred when PMNLs were incubated with 1, 10, 20, 1000 and 200 micrograms/ml concentrations of Adriamycin. Preincubation of the PMNLs with vitamin E afforded partial protection at lower concentrations. The total uptake at 3, 10 and 20 min of methyl 3H thymidine labeled S. aureus by control PMNL was significantly greater than by PMNLs exposed for 60 min to 100 micrograms/ml of Adriamycin. Intracellular Adriamycin concentrations increased in a dose dependent fashion. Viability of cells before and after incubation was 95%. Adriamycin inhibited oxidative metabolic and functional activity of PMNLs in a dose related manner. Preincubation of PMNLs with vitamin E partially protected them from the effects of Adriamycin.

摘要

测定了不同浓度的阿霉素单独作用以及阿霉素与维生素E联合作用对人多形核白细胞（PMNL）功能的影响。将等分的PMNL在37℃下与生理盐水或维生素E孵育，然后暴露于阿霉素。当PMNL与浓度为1、10、20、1000和200微克/毫升的阿霉素孵育时，酵母聚糖刺激的PMNL对1-14C葡萄糖的利用和氧消耗显著降低。PMNL与维生素E预孵育在较低浓度时提供了部分保护。对照PMNL在3、10和20分钟时对3H甲基胸苷标记的金黄色葡萄球菌的总摄取量显著高于暴露于100微克/毫升阿霉素60分钟的PMNL。细胞内阿霉素浓度呈剂量依赖性增加。孵育前后细胞的活力为95%。阿霉素以剂量相关的方式抑制PMNL的氧化代谢和功能活性。PMNL与维生素E预孵育可部分保护它们免受阿霉素的影响。