Ross J, Pizarro A
J Mol Biol. 1983 Jul 5;167(3):607-17. doi: 10.1016/s0022-2836(83)80101-6.
We have tested the hypothesis of Winslow & Ingram (1966) that the steady-state levels of human beta and delta globin proteins are determined, in part, by the stabilities of their respective messenger RNAs. Nucleated bone marrow cells were cultured under different RNA "chase" conditions, and samples were harvested at intervals thereafter. The quantities of beta and delta globin mRNA sequences in total RNA from these cells were measured by solution hybridization with specific 32P-labeled DNA probes. The average half-lives of beta and delta globin mRNAs are 16.5 and 4.5 hours, respectively. Both mRNAs are polyadenylated. The rapid turnover of delta globin mRNA accounts, at least in part, for the low level of delta globin mRNA in non-nucleated peripheral blood reticulocytes. The possibility that the rate of mRNA decay is determined by nucleotide sequence signals located in the 3' untranslated region is discussed.
我们检验了温斯洛和英格拉姆（1966年）提出的假说，即人类β和δ珠蛋白的稳态水平部分由其各自信使核糖核酸的稳定性决定。有核骨髓细胞在不同的RNA“追踪”条件下培养，此后每隔一段时间采集样本。通过与特定的32P标记DNA探针进行溶液杂交，测量这些细胞总RNA中β和δ珠蛋白信使核糖核酸序列的量。β和δ珠蛋白信使核糖核酸的平均半衰期分别为16.5小时和4.5小时。两种信使核糖核酸都进行了多聚腺苷酸化。δ珠蛋白信使核糖核酸的快速周转至少部分解释了无核外周血网织红细胞中δ珠蛋白信使核糖核酸水平较低的原因。本文还讨论了信使核糖核酸衰变速率由位于3'非翻译区的核苷酸序列信号决定的可能性。
