Rubin H, Halim M
San Diego Institute of Molecular Biology, CA 92121.
Cell Mol Biol Res. 1993;39(1):73-80.
We report here that when alpha- or beta-globin mRNAs were hybridized with their alpha- or beta-complementary DNAs, respectively, and then translated in rabbit reticulocyte lysates, globin synthesis as well as shortening of the poly(A) tail were much less than that observed with nonhybridized mRNA. Furthermore, when the hybrid (alpha-globin mRNA/alpha-cDNA) was translated in the presence of nonhybrid (alpha-globin mRNA), the estimated average shortening of the poly(A) tails of the hybrid and the nonhybrid were 8% and 40%, respectively. Similar results were obtained when a hybridized beta-globin mRNA with beta-cDNA was incubated in the same translation mixture with beta-globin mRNA. No globin synthesis occurred in the mixtures containing the cDNA-mRNA hybrids only. In the mixtures containing both the hybrid and the nonhybridized mRNAs, globin was synthesized at a rate similar to that observed in a translation reaction mixture alone, that is, in the absence of any hybrid.
我们在此报告，当α-或β-珠蛋白mRNA分别与其α-或β-互补DNA杂交，然后在兔网织红细胞裂解物中进行翻译时，珠蛋白合成以及多聚腺苷酸（poly(A)）尾巴的缩短程度远低于未杂交的mRNA。此外，当杂交体（α-珠蛋白mRNA/α-cDNA）在非杂交体（α-珠蛋白mRNA）存在的情况下进行翻译时，杂交体和非杂交体的多聚腺苷酸尾巴估计平均缩短率分别为8%和40%。当杂交的β-珠蛋白mRNA与β-cDNA在与β-珠蛋白mRNA相同的翻译混合物中孵育时，也获得了类似的结果。仅含有cDNA-mRNA杂交体的混合物中未发生珠蛋白合成。在同时含有杂交体和未杂交mRNA的混合物中，珠蛋白的合成速率与仅在翻译反应混合物中观察到的速率相似，即在不存在任何杂交体的情况下。
