The distribution of molecular classes of phosphatidylglycerol in the membrane of Acholeplasma laidlawii.

A double-label technique has been applied to study the distribution of different molecular classes of phosphatidylglycerol in the membrane of Acholeplasma laidlawii. After growth on oleic acid, 16% of the total phosphatidylglycerol contains two oleic acid residues and 84% contains one oleic acid and one saturated fatty acid. The dioleoyl phosphatidylglycerol is present in equal amounts in the outer and the inner layer of the membrane. Phosphatidylglycerol which is associated with membrane proteins consists exclusively of the class containing only one oleic acid.