Mersmann G, Becker R, Buddecke E
Biochim Biophys Acta. 1978 Jul 7;525(1):154-61. doi: 10.1016/0005-2744(78)90209-7.
alpha-Mannosidase (alpha-D-mannoside mannohydrolase, EC 3.2.1.24) from pig kidney has been shown to exist in multiple forms differing in their capability to be endocytosed by alpha-mannosidase deficient cultured cells. A method is presented to evaluate the amount of "uptake" forms in different preparations of the enzyme. Preparations with different rates of uptake were shown to contain different amount of "uptake" forms and "non-uptake" forms. The content of "uptake" forms in a preparation was identical with that of enzyme molecules bearing a phosphorylated carbohydrate group necessary for the recognition by cell surface receptors.
猪肾α-甘露糖苷酶（α-D-甘露糖苷甘露水解酶，EC 3.2.1.24）已被证明以多种形式存在，这些形式在被α-甘露糖苷酶缺陷的培养细胞内吞的能力上有所不同。本文介绍了一种评估该酶不同制剂中“可摄取”形式含量的方法。结果表明，具有不同摄取速率的制剂含有不同量的“可摄取”形式和“不可摄取”形式。制剂中“可摄取”形式的含量与带有细胞表面受体识别所需磷酸化碳水化合物基团的酶分子含量相同。
