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不同来源RNA的电滞后现象及线粒体整合

Electric hysteresis and mitochondrial incorporation of RNAs from different sources.

作者信息

Galzigna L, Garbin L

出版信息

Biochimie. 1978;60(4):345-51. doi: 10.1016/s0300-9084(78)80667-1.

DOI:10.1016/s0300-9084(78)80667-1
PMID:687674
Abstract

The paper reports the characteristics of four different RNAs from yeast, Torula and calf thymus of molecular weight ranging between 15,000 and 30,000. The gel-filtration behaviour with aqueous and partially qqueous solvents is studied together with the response of the four RNAs to static electric fields of strength ranging between 20 and 35 kV/cm. The relationship between molecular weight and extent of electric hysteresis is linear for all RNAs, while tRNA slightly deviates from such a relationship. The ability of the RNAs to permeate biological mebranes or bind membrane components such as lecithins is studied with rat liver mitochondria and a two-phase system with egg lecithin dissolved in the organic phase and RNA in the aqueous one. There is no apparent relationship between molecular weight of the RNAs and their ability to interact with biological membranes.

摘要

该论文报道了来自酵母、球拟酵母和小牛胸腺的四种不同RNA的特性,其分子量在15,000至30,000之间。研究了这四种RNA在水性和部分水性溶剂中的凝胶过滤行为,以及它们对强度在20至35 kV/cm之间的静电场的响应。所有RNA的分子量与电滞现象程度之间的关系呈线性,而tRNA略微偏离这种关系。利用大鼠肝线粒体以及一个两相系统研究了这些RNA渗透生物膜或结合膜成分(如卵磷脂)的能力,该两相系统中有机相溶解有鸡蛋卵磷脂,水相中含有RNA。RNA的分子量与其与生物膜相互作用的能力之间没有明显关系。

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1
Electric hysteresis and mitochondrial incorporation of RNAs from different sources.不同来源RNA的电滞后现象及线粒体整合
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