一种在大鼠中诱导致命性、IgE 介导的支气管和心血管过敏反应的新方法。

A new method for inducing fatal, IgE-mediated, bronchial and cardiovascular anaphylaxis in the rat.

作者信息

Ufkes J G, Ottenhof M, Aalberse R C

出版信息

J Pharmacol Methods. 1983 May;9(3):175-81. doi: 10.1016/0160-5402(83)90036-0.

Abstract

Brown-Norway rats, sensitized with trinitrophenyl (TNP) haptenized ovalbumin and AIPO4 as adjuvant 12 days before, were challenged with trinitrophenyl haptenized bovine serum albumin intravenously, while lung function (Vt, V, Ppl, Fres, Cdyn, and Rl) and cardiovascular function (BP and Fheart) were measured continuously. This resulted in a highly reproducible, plasma IgE-antiTNP related, immediate anaphylactic response characterized by a short-lasting (8-10 min) bronchoconstriction, together with a long-lasting fall in blood pressure. All rats died in shock within 21-150 min. This method is simple and appeared to be highly reproducible and therefore suitable to screen or study antiallergic drugs in vivo.

摘要

在12天前用三硝基苯基（TNP）半抗原化卵清蛋白和磷酸铝作为佐剂致敏的棕色挪威大鼠，通过静脉注射三硝基苯基半抗原化牛血清白蛋白进行激发，同时连续测量肺功能（潮气量、肺活量、胸膜腔内压、呼吸频率、动态顺应性和气道阻力）和心血管功能（血压和心率）。这导致了一种高度可重复的、与血浆IgE - 抗TNP相关的速发型过敏反应，其特征为短暂的（8 - 10分钟）支气管收缩以及血压的长期下降。所有大鼠在21 - 150分钟内死于休克。该方法简单，似乎具有高度可重复性，因此适合在体内筛选或研究抗过敏药物。