磷酸烯醇式丙酮酸羧激酶在布氏布氏锥虫前循环期糖体中的存在及作用

Occurrence and role of phosphoenolpyruvate carboxykinase in procyclic Trypanosoma brucei brucei glycosomes.

作者信息

Broman K, Knupfer A L, Ropars M, Deshusses J

出版信息

Mol Biochem Parasitol. 1983 May;8(1):79-87. doi: 10.1016/0166-6851(83)90036-1.

Abstract

Phosphoenolpyruvate carboxykinase (EC 4.1.1.32) was detected in a particulate fraction of Trypanosoma brucei brucei procyclic culture form. It requires ADP rather than GDP for activity in the direction of carboxylation and is located in the glycosomes. Since phosphoenolpyruvate can serve to furnish ATP for glycolysis and can promote 3-phosphoglycerate or 1,3-bisphosphoglycerate formation without simultaneous alpha-glycerophosphate production, we suggest that the glycosomal phosphoenolpyruvate carboxykinase-malate dehydrogenase tandem contributes to ATP regeneration and NADH re-oxidation in the glycosome, and regulates alpha-glycerophosphate production.

摘要

在布氏布氏锥虫前循环型培养物的颗粒部分中检测到磷酸烯醇丙酮酸羧激酶（EC 4.1.1.32）。在羧化方向上，其活性需要ADP而非GDP，并且位于糖体中。由于磷酸烯醇丙酮酸可用于为糖酵解提供ATP，且可促进3-磷酸甘油酸或1,3-二磷酸甘油酸的形成，而不同时产生α-甘油磷酸，我们认为糖体中的磷酸烯醇丙酮酸羧激酶-苹果酸脱氢酶串联有助于糖体中的ATP再生和NADH再氧化，并调节α-甘油磷酸的产生。