六种线虫、一种绦虫和一种吸虫中的核糖核苷酸水平。

Ribonucleotide levels in six nematodes, a cestode and a trematode.

作者信息

Darwish A A, Sangster N C, Prichard R K

出版信息

Mol Biochem Parasitol. 1983 Jun;8(2):109-17. doi: 10.1016/0166-6851(83)90003-8.

Abstract

The levels of ribonucleotides in the nematodes Haemonchus contortus, Ostertagia circumcincta, Trichostrongylus colubriformis, Nippostrongylus brasiliensis, Ascaris suum and Stephanurus dentatus, the cestode Moniezia expansa and the trematode Fasciola hepatica were measured by high pressure liquid chromatography. The adenylate charge, ATP and total adenine nucleotide levels were in general agreement with those determined enzymatically and with published data. UDP and guanine nucleotide levels were found to be higher than those present in mammals and previously reported for helminth parasites. Cytidine nucleotide levels varied considerably between parasite species. On the other hand inosine nucleotides were present at low concentrations in all species. Adenylate and guanylate charges were similar.

摘要

通过高压液相色谱法测定了捻转血矛线虫、环形奥斯特线虫、蛇形毛圆线虫、巴西日圆线虫、猪蛔虫和有齿冠尾线虫等线虫、扩展莫尼茨绦虫和肝片吸虫等吸虫中核糖核苷酸的水平。腺苷酸电荷、ATP和总腺嘌呤核苷酸水平总体上与酶法测定的结果以及已发表的数据一致。发现UDP和鸟嘌呤核苷酸水平高于哺乳动物中的水平，且高于先前报道的蠕虫寄生虫中的水平。胞嘧啶核苷酸水平在不同寄生虫物种之间差异很大。另一方面，肌苷核苷酸在所有物种中的浓度都很低。腺苷酸和鸟苷酸电荷相似。