Polson A, Gelderblom D, van der Merwe K J
Prep Biochem. 1983;13(2):137-59. doi: 10.1080/00327488308068744.
The H tube electrophoresis technique was used for isolating human IgG by the net effect of electrophoresis and electro-endosmosis in agarose gel containing human serum. In a similar experiment human serum albumin was purified by extracting the agarose gel slice containing the albumin following zone electrophoresis of serum in agarose. By taking advantage of the relatively high reverse electro-endosmotic flow compared to the migration of IgG in agarose gel, sheep anti chicken immunoglobulin was fractionated into active and inactive portions. It was estimated that 90% of the nonspecific IgG could be eliminated in this manner. The specific immunoglobulin fraction showed a higher mobility to the positive electrode than the bulk of the non specific IgG. The H tube proved to be ideal for zone electrophoresis in concentration gradients of inert substances. For this purpose sugar gradients were used for electrophoretic separation of proteins and viruses which are not affected by high osmotic pressures but, for separating animal cells it was necessary to use Ficoll gradients. A mixture of yellow Atypical Tomato Mosaic Virus and the haemocyanin of Burnupena cincta were separated into discrete zones and chicken IgY anti haemocyanin was subfractionated. The specific fraction showed a lower migration rate to the positive pole than the bulk of the non specific material.
采用H管电泳技术，利用人血清琼脂糖凝胶中电泳和电渗的净效应分离人IgG。在类似实验中，通过对琼脂糖凝胶中血清进行区带电泳后提取含白蛋白的琼脂糖凝胶切片，来纯化人血清白蛋白。利用与IgG在琼脂糖凝胶中迁移相比相对较高的反向电渗流，将羊抗鸡免疫球蛋白分离为活性和非活性部分。据估计，以这种方式可消除90%的非特异性IgG。特异性免疫球蛋白组分向正极的迁移率高于大部分非特异性IgG。H管被证明是在惰性物质浓度梯度中进行区带电泳的理想工具。为此，糖梯度用于蛋白质和病毒的电泳分离，它们不受高渗透压影响，但对于分离动物细胞，则必须使用聚蔗糖梯度。黄色非典型番茄花叶病毒和带纹滨蟹血蓝蛋白的混合物被分离成离散区带，鸡抗血蓝蛋白IgY被进一步细分。特异性组分向正极的迁移率低于大部分非特异性物质。
