超声对胚胎发育的影响。

The influence of ultrasound on embryonic development.

作者信息

Barnett S B

出版信息

Ultrasound Med Biol. 1983 Jan-Feb;9(1):19-24. doi: 10.1016/0301-5629(83)90105-9.

Abstract

The chick embryo provides a convenient experimental subject to assess the effects of ultrasound on a developing biological system. Insonating the whole embryo prior to organogenesis with spatial peak temporal average intensities of 4.5 W/cm2 pulsed, and 100 W/cm2 continuous wave focused, produced no lethal effect or observable developmental changes during three days following the treatment. Any bio-effect which may have resulted from the interaction of ultrasound at the cellular level was not severe enough to bring about irreversible gross physical abnormalities in the developing embryo. The effects of standing waves or other extraneous physical factors were considered to be greatly reduced by irradiating embryos "in ovo" rather than in glass dishes following explantation.

摘要

鸡胚为评估超声对发育中的生物系统的影响提供了一个便利的实验对象。在器官发生之前，用4.5W/cm²的脉冲空间峰值时间平均强度和100W/cm²的连续波聚焦强度对整个胚胎进行超声照射，在处理后的三天内未产生致死效应或可观察到的发育变化。超声在细胞水平相互作用可能产生的任何生物效应都不够严重，不足以在发育中的胚胎中导致不可逆的明显身体异常。通过“在卵内”照射胚胎，而不是在移出后置于玻璃培养皿中照射，驻波或其他外部物理因素的影响被认为会大大降低。