小鼠脉冲超声致畸性检测。

Testing for the teratogenicity of pulsed ultrasound in mice.

作者信息

Child S Z, Carstensen E L, Gates A H, Hall W J

机构信息

Rochester Center for Biomedical Ultrasound, University of Rochester, NY 14627.

出版信息

Ultrasound Med Biol. 1988;14(6):493-8. doi: 10.1016/0301-5629(88)90110-x.

DOI:10.1016/0301-5629(88)90110-x

PMID:3227572

Abstract

Our replicate of a study by Takabayashi et al. (1981) Effects of pulse-wave ultrasonic irradiation on mouse embryo. Cho-Onpa Igaku (Supersonic Medicine) 8, 286-288 failed to show any effects of exposure in utero of mice to spatial average, temporal maximum intensities of 60 W/cm2. Fetuses were exposed at 8 days post fertilization and assessed at 18 days for fetal weight, resorptions, premature deaths, and malformations. Carrier frequency in the pulse was 2 MHz. Pulse lengths of 10 microseconds and pulse repetition frequencies of 1000 and 200 Hz yielded spatial average, temporal average intensities of 0.6 and 0.1 W/cm2. Total exposure time was 5 min. Our results provide no basis to conclude that conditions relevant to human fetal monitoring will cause developmental, externally visible anomalies in mice exposed as 8-day fetuses.

摘要

我们重复了Takabayashi等人（1981年）的一项研究“脉冲波超声照射对小鼠胚胎的影响”。《超音波医学》8卷，第286 - 288页，该研究未能显示子宫内小鼠暴露于空间平均、时间最大强度为60 W/cm²的超声下有任何影响。在受精后8天对胎儿进行暴露，并在18天时评估胎儿体重、吸收情况、早产死亡和畸形情况。脉冲的载波频率为2 MHz。10微秒的脉冲长度以及1000 Hz和200 Hz的脉冲重复频率产生的空间平均、时间平均强度分别为0.6 W/cm²和0.1 W/cm²。总暴露时间为5分钟。我们的结果没有依据得出与人类胎儿监测相关的条件会在8天胎儿期暴露的小鼠中导致发育性、外部可见异常的结论。