Ceri H, Wright J A
Can J Microbiol. 1977 Jun;23(6):835-9. doi: 10.1139/m77-124.
Chinese hamster ovary cells cultured in the presence of phenethyl alcohol exhibit obvious changes in cell surface galactose and galactosamine glycoproteins as determined by the galactose-oxidase[3H]borohydride technique and SDS gel electrophoresis. Cells pretreated with phenethyl alcohol (drug was removed before infection) were not as effective as hosts for vesicular stomatitis virus as untreated cultures. A minimum pretreatment time with 0.1% phenethyl alcohol of about 8 h was required before a reduction in virus growth was observed. It is proposed that phenethyl alcohol pretreatment as outlined in this report leads to a modification of the host cellular membrane resulting in the inhibition of virus replication.
用半乳糖氧化酶-[³H]硼氢化钠技术和十二烷基硫酸钠凝胶电泳法测定，在苯乙醇存在下培养的中国仓鼠卵巢细胞，其细胞表面半乳糖和半乳糖胺糖蛋白出现明显变化。用苯乙醇预处理的细胞（感染前去除药物）作为水疱性口炎病毒的宿主，其效果不如未处理的培养物。在观察到病毒生长减少之前，需要用0.1%苯乙醇进行至少约8小时的最低预处理时间。据推测，本报告中所述的苯乙醇预处理会导致宿主细胞膜发生改变，从而抑制病毒复制。
