Chemotaxis of resident, elicited and immunologically activated murine peritoneal macrophages.

The in vitro chemotactic response of peritoneal macrophages fourteen days after immunization with BCG was greater than that of macrophages from control mice. Peritoneal macrophages from mice treated with other agents which enhance bactericidal activity, and macrophages induced with proteose-peptone were less responsive to chemotactic stimuli than resident macrophages. The addition of PPD or PHA lymphokines to the peritoneal cells from BCG injected mice depressed the chemotactic response, but increased unstimulated migration. PPD had no effect on the migration of resident macrophages, but PHA lymphokines depressed the chemotactic activity of these cells.