Detroy R W, DeMarini D M, Still P E
Can J Microbiol. 1978 Aug;24(8):947-53. doi: 10.1139/m78-157.
Carbon-nitrogen ratio experiments indicate that limiting nutrition not only hinders Penicillium stoloniferum host proliferation but reduces total PsV-F and PsV-S virus replication. Results of C-N experiments show a pH-induced autolysis and virus release at minimal C levels. Maximal PsV-F levels and biomass were obtained with glucose and sucrose as C sources. Oleic acid also yielded high biomass and PsV-F yields. Yeast extract was an excellent N source; 2.83 g dry weight biomass and 87 A260 units PsV-F after 96 h of growth. Other nitrogen sources, including amino acids, supported only minimal growth and virus replication. The autolysis phenomenon is pH, not viral-induced. High C and N will support maximal growth and unrestricted virus replication with no cellular lysis. Under low C growth conditions, the replication of PsV-S is favored coupled with high pH and autolysis.
碳氮比实验表明，营养限制不仅会阻碍匐枝青霉宿主的增殖，还会减少总PsV - F和PsV - S病毒的复制。碳氮实验结果表明，在最低碳水平下会发生pH诱导的自溶和病毒释放。以葡萄糖和蔗糖作为碳源时可获得最大的PsV - F水平和生物量。油酸也产生了较高的生物量和PsV - F产量。酵母提取物是一种优质氮源；生长96小时后，干重生物量为2.83克，PsV - F为87个A260单位。其他氮源，包括氨基酸，仅支持最低限度的生长和病毒复制。自溶现象是由pH引起的，而非病毒诱导。高碳和高氮将支持最大生长和不受限制的病毒复制，且不会发生细胞裂解。在低碳生长条件下，PsV - S的复制在高pH和自溶的情况下更受青睐。
