Detroy R W, Still P E
J Gen Microbiol. 1976 Jan;92(1):167-74. doi: 10.1099/00221287-92-1-167.
Penicillium stoloniferum NRRL5267 contains two electrophoretically distinct viruses (PsV-F and PsV-S). An in vivo system was developed to test whether a number of fungal metabolites had antiviral properties on PsV-F replication in O.erties on PsV-F replication in P. stoloniferum. Preliminary results indicated that the mycotoxin patulin can block mycovirus replication. Portions of 48 h mycelium were incubated in the presence of varying levels of patulin, and after an additional 48 h incubation, PsV-F content was measured in E260 units by polyacrylamide gel electrophoresis. Patulin at 11, 16 and 20 mug/mg dry wt mycelia blocked PsV-F replication 26, 61 and 71%, respectively, compared with untreated controls. At these levels, host biomass RNA and protein synthesis were minimally affected. No-proliferating fungal mycelium is capable of continued support of PsV-F replication, which is sensitive to patulin. Apparently, inhibitory doses of patulin stimulated PsV-S replication during this 48 h incubation. The preferential action of patulin may arise from metabolite binding to functional enzymes required for virus replication.
匍匐青霉NRRL5267含有两种电泳性质不同的病毒（PsV-F和PsV-S）。开发了一种体内系统来测试多种真菌代谢产物对匍匐青霉中PsV-F复制是否具有抗病毒特性。初步结果表明，霉菌毒素棒曲霉素可阻断真菌病毒复制。将48小时的菌丝体部分在不同水平的棒曲霉素存在下孵育，再额外孵育48小时后，通过聚丙烯酰胺凝胶电泳以E260单位测量PsV-F含量。与未处理的对照相比，每毫克干重菌丝体中11、16和20微克的棒曲霉素分别阻断了26%、61%和71%的PsV-F复制。在这些水平下，宿主生物量RNA和蛋白质合成受到的影响最小。不增殖的真菌菌丝体能够持续支持对棒曲霉素敏感的PsV-F复制。显然，在这48小时的孵育过程中，抑制剂量的棒曲霉素刺激了PsV-S复制。棒曲霉素的优先作用可能源于代谢产物与病毒复制所需的功能酶结合。
