Ochi Y
Gan To Kagaku Ryoho. 1983 Feb;10(2 Pt 2):585-94.
The purified CEA preparation by anti-alpha 1-acid glycoprotein (AG) chromatography shows specific binding with both auto-antibodies for CEA in cancerous patients and the monoclonal antibody for CEA. By this method CEA purification is possible. CEA preparation obtained from CEA producing cell line and CEA producing tumor grafted in nude mice contain AG antigenic determinants. Both tumors also contain free AG (Mr, 50,000). When tryptic hydrolysis of purified CEA preparation is performed, AG-like protein (Mr, 30,000) is released. The carbohydrate composition and amino acid content of both CEA and AG have a significant similarity. We postulate that CEA may be a biosynthetic precursor of AG based on differences in binding affinities between CEA with anti-CEA and with anti-AG.
通过抗α1-酸性糖蛋白（AG）色谱法纯化的癌胚抗原（CEA）制剂，显示出与癌症患者体内针对CEA的自身抗体以及CEA单克隆抗体均有特异性结合。通过这种方法可以实现CEA的纯化。从产生CEA的细胞系以及移植到裸鼠体内的产生CEA的肿瘤中获得的CEA制剂含有AG抗原决定簇。这两种肿瘤中还含有游离的AG（分子量50,000）。对纯化的CEA制剂进行胰蛋白酶水解时，会释放出类似AG的蛋白（分子量30,000）。CEA和AG的碳水化合物组成及氨基酸含量有显著相似性。基于CEA与抗CEA及抗AG之间结合亲和力的差异，我们推测CEA可能是AG的生物合成前体。
