Hart I R, Fidler I F
Cancer Res. 1978 Oct;38(10):3218-24.
An in vitro quantitative assay of tumor cell invasion is described. The assay measures the rate at which [125I]iododeoxyuridine-labeled tumor cells migrate through the chorioallantoic membrane of developing chicken embryos. Invasion chambers were prepared from amber latex cylinders, chorioallantoic membrane, and thread ligatures. The chambers were placed in glass vials to rest on discs of photographic sponge immersed in tissue culture medium. Labeled tumor cells were added to the chamber, and the vial was incubated at 37 degrees. At various time points individual chambers were monitored to determine the number of viable, labeled cells that had passed through the membrane and were present in the medium, on the glass vial, or in the sponge. The application of the assay may be of use in delineating the mechanisms of tumor cell invasion.
本文描述了一种肿瘤细胞侵袭的体外定量检测方法。该检测方法测量[125I]碘脱氧尿苷标记的肿瘤细胞穿过发育中鸡胚绒毛尿囊膜的迁移速率。侵袭小室由琥珀色乳胶圆柱体、绒毛尿囊膜和丝线结扎制成。将小室置于玻璃小瓶中，使其靠在浸于组织培养基中的照相海绵圆盘上。将标记的肿瘤细胞加入小室，小瓶于37℃孵育。在不同时间点监测各个小室，以确定穿过膜并存在于培养基、玻璃小瓶或海绵中的存活标记细胞数量。该检测方法的应用可能有助于阐明肿瘤细胞侵袭的机制。
